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KMID : 0644120080210020049
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Journal of Oriental Gynecology
2008 Volume.21 No. 2 p.49 ~ p.58
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Effects of Astragalus membranaceus Extract on Cell Cycle and Apoptosis of Human Leiomyoma Cell
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Park Jun-Gyou
Lee Jin-Moo
Lee Chang-Hoon
Cho Jung-Hoon
Jang Jun-Bock
Lee Kyung-Sub
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Abstract
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Purpose: This study was conducted to investigate the effects of Astragalus membranaceus extract solution on cell cycle regulation and apoptosis of human leiomyomal cell.
Methods: The leiomyoma cell of patients was used in the study, and we administered the extract solution of Astragalus membranaceus concentration at 1, 10㎎/㎖ to the leiomyoma cell for 48 hours. We used flow cytometry and western blotting to confirm cell cycle and apoptosis.
Results: In flow cytometry, G1 phase of the 1㎎/㎖ and 10㎎/㎖ group was shortened and S phase of the 1㎎/㎖ and 10㎎/㎖ group was increased. Cycline D1 expression increased in 1, 10㎎/㎖ group. Bcl-2 expression decreased in 1, 10㎎/㎖ groups than control group. And Bax expression that regulated cell apoptosis more increased in 1, 10㎎/㎖ group than control group. VEGF expression rised in higher Astragalus membranaceus concentration group.
Conclusion: This study suggest that Astragalus membranaceus might induce cell apoptosis of leiomyoma cell and shorten cell cycle. And Astragalus membranaceus would not have the regulation effect of cell cycle.
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KEYWORD
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Astragalus membranaceus, leiomyoma, apoptosis, cell cycle
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